Fractionation and isolation of polyphenols from Aronia melanocarpa by countercurrent and membrane chromatography

verfasst von

T. Esatbeyoglu, M. Rodríguez-Werner, P. Winterhalter

Abstract

In the current study, the fractionation and isolation of polyphenols from Aronia melanocarpa has been performed with two different chromatographic techniques on a large scale. On the one hand, the fractionation of polyphenols such as anthocyanins, phenolic acids, quercetin–glycosides and flavanons from A. melanocarpa pomace was done by high-speed countercurrent chromatography and low-speed rotary countercurrent chromatography. On the other hand, the preseparation of A. melanocarpa extracts from pomace and juice in an anthocyanin and a co-pigment fraction was carried out by membrane chromatography after removing the polymeric procyanidins by precipitation with ethanol. Afterward, the separation and isolation of anthocyanins and co-pigments were done by preparative countercurrent chromatography. Purity control and identification of the isolated compounds were made by HPLC–PDA, HPLC–ESI-MS

ⁿ and

¹H- as well as

¹³C-NMR spectroscopy. Various compounds, e.g., chlorogenic acids, isorhamnetin-, apigenin-, luteolin- and taxifolin-derivatives, are described for A. melanocarpa for the first time.

Organisationseinheit(en)

Institut für Lebensmittelwissenschaft und Humanernährung
Molekulare Lebensmittelchemie und -entwicklung

Typ

Artikel

Journal

European Food Research and Technology

Band

243

Seiten

1261-1275

Anzahl der Seiten

ISSN

1438-2377

Publikationsdatum

01.07.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

ASJC Scopus Sachgebiete

Biotechnologie, Lebensmittelwissenschaften, Chemie (insg.), Biochemie, Wirtschaftsingenieurwesen und Fertigungstechnik

Elektronische Version(en)

https://doi.org/10.1007/s00217-016-2837-3 (Zugang: Geschlossen)