Dryopteris juxtapostia Root and Shoot

Determination of Phytochemicals; Antioxidant, Anti-Inflammatory, and Hepatoprotective Effects; and Toxicity Assessment

authored by

Abida Rani, Muhammad Uzair, Shehbaz Ali, Muhammad Qamar, Naveed Ahmad, Malik Waseem Abbas, Tuba Esatbeyoglu

Abstract

An estimated 450 species of

Dryopteris in the Dryoperidaceae family grow in Japan, North and South Korea, China, Pakistan, and Kashmir. This genus has been reported to have biological capabilities; however, research has been conducted on

Dryopteris juxtapostia. Therefore, with the present study, we aimed to exploring the biological potential of

D. juxtapostia root and shoot extracts. We extracted dichloromethane and methanol separately from the roots and shoots of

D. juxtapostia. Antioxidant activity was determined using DPPH, FRAP, and H

2 assays, and anti-inflammatory activities were evaluated using both in vitro (antiurease activity) and in vivo (carrageenan- and formaldehyde-induced paw edema) studies. Toxicity was evaluated by adopting a brine shrimp lethality assay followed by determination of cytotoxic activity using an MTT assay. Hepatoprotective effects of active crude extracts were examined in rats. Activity-bearing compounds were tentatively identified using LC-ESI-MS/MS analysis. Results suggested that

D. juxtapostia root dichloromethane extract exhibited better antioxidant (DPPH, IC

50 of 42.0 µg/mL; FRAP, 46.2 mmol/g; H

2, 71% inhibition), anti-inflammatory (urease inhibition, 56.7% at 50 µg/mL; carrageenan-induced edema inhibition, 61.7% at 200 µg/mL; formaldehyde-induced edema inhibition, 67.3% at 200 µg/mL), brine shrimp % mortality (100% at 1000 µg/mL), and cytotoxic (HeLa cancer, IC

50 of 17.1 µg/mL; prostate cancer (PC3), IC

50 of 45.2 µg/mL) effects than

D. juxtapostia root methanol extract.

D. juxtapostia shoot dichloromethane and methanol extracts exhibited non-influential activity in all biological assays and were not selected for hepatoprotective study.

D. juxtapostia root methanol extract showed improvement in hepatic cell structure and low cellular infiltration but, in contrast the dichloromethane extract, did not show any significant improvement in hepatocyte morphology, cellular infiltration, or necrosis of hepatocytes in comparison to the positive control, i.e., paracetamol. LC-ESI-MS/MS analysis showed the presence of albaspidin PP, 3-methylbutyryl-phloroglucinol, flavaspidic acid AB and BB, filixic acid ABA and ABB, tris-desaspidin BBB, tris-paraaspidin BBB, tetra-flavaspidic BBBB, tetra-albaspidin BBBB, and kaempferol-3-

O-glucoside in the dichloromethane extract, whereas kaempferol, catechin, epicatechin, quinic acid, liquitrigenin, and quercetin 7-

O-galactoside in were detected in the methanol extract, along with all the compounds detected in the dichloromethane extract. Hence,

D. juxtapostia is safe, alongside other species of this genus, although detailed safety assessment of each isolated compound is obligatory during drug discovery.

Organisation(s)

Institute of Food Science and Human Nutrition
Molecular Food Chemistry and Food Development

External Organisation(s)

Bahauddin Zakariya University
Khwaja Fareed University of Engineering and Information Technology (KFUEIT)
Multan Medical and Dental College (MMDC)

Type

Article

Journal

Antioxidants

Volume

ISSN

2076-3921

Publication date

27.08.2022

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Food Science, Molecular Biology, Physiology, Biochemistry, Clinical Biochemistry, Cell Biology

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.3390/antiox11091670 (Access: Open)