Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 - studies in cultured HT-29 cells and mice

authored by

A.E. Wagner, C. Sturm, S. Piegholdt, I.M.A. Wolf, T. Esatbeyoglu, G.R. De Nicola, R. Iori, G. Rimbach

Abstract

In this study, the effect of myrosinase-treated glucoerucin (GER+MYR), which releases the isothiocyanate (ITC) erucin, on heme oxygenase 1 (HO-1) gene expression and Nrf2 signaling was investigated in vitro in cultured cells and in vivo in mice. Treatment of HT-29 cells with GER+MYR resulted in a significant increase in the mRNA and protein levels of nuclear Nrf2 and HO-1. GER+MYR was more potent at enhancing the nuclear Nrf2 levels than were the following myrosinase-treated glucosinolates: sinigrin, glucoraphanin and gluconasturtiin, which are the precursors of allyl-ITC, R-sulforaphane and 2-phenylethyl ITC, respectively. GER+MYR also significantly induced HO-1 gene expression in the mouse intestinal mucosae and liver but not in the brain. Mechanistic studies suggest that GER+MYR induces Nrf2 via ERK1/2-, p38- and JNK-dependent signal transduction pathways. The GER+MYR-mediated increase in HO-1 expression is primarily attributable to p38 signaling.

Organisation(s)

Institute of Food Science and Human Nutrition
Molecular Food Chemistry and Food Development

Type

Article

Journal

Journal of Nutritional Biochemistry

Volume

26

Pages

661-666

No. of pages

6

ISSN

0955-2863

Publication date

01.06.2015

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism, Biochemistry, Molecular Biology, Nutrition and Dietetics, Clinical Biochemistry

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.1016/j.jnutbio.2015.01.004 (Access: Closed)