Fractionation and isolation of polyphenols from Aronia melanocarpa by countercurrent and membrane chromatography

authored by

T. Esatbeyoglu, M. Rodríguez-Werner, P. Winterhalter

Abstract

In the current study, the fractionation and isolation of polyphenols from Aronia melanocarpa has been performed with two different chromatographic techniques on a large scale. On the one hand, the fractionation of polyphenols such as anthocyanins, phenolic acids, quercetin–glycosides and flavanons from A. melanocarpa pomace was done by high-speed countercurrent chromatography and low-speed rotary countercurrent chromatography. On the other hand, the preseparation of A. melanocarpa extracts from pomace and juice in an anthocyanin and a co-pigment fraction was carried out by membrane chromatography after removing the polymeric procyanidins by precipitation with ethanol. Afterward, the separation and isolation of anthocyanins and co-pigments were done by preparative countercurrent chromatography. Purity control and identification of the isolated compounds were made by HPLC–PDA, HPLC–ESI-MS

ⁿ and

¹H- as well as

¹³C-NMR spectroscopy. Various compounds, e.g., chlorogenic acids, isorhamnetin-, apigenin-, luteolin- and taxifolin-derivatives, are described for A. melanocarpa for the first time.

Organisation(s)

Institute of Food Science and Human Nutrition
Molecular Food Chemistry and Food Development

Type

Article

Journal

European Food Research and Technology

Volume

243

Pages

1261-1275

No. of pages

15

ISSN

1438-2377

Publication date

01.07.2017

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Food Science, Chemistry(all), Biochemistry, Industrial and Manufacturing Engineering

Electronic version(s)

https://doi.org/10.1007/s00217-016-2837-3 (Access: Closed)