Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 - studies in cultured HT-29 cells and mice

verfasst von

A.E. Wagner, C. Sturm, S. Piegholdt, I.M.A. Wolf, T. Esatbeyoglu, G.R. De Nicola, R. Iori, G. Rimbach

Abstract

In this study, the effect of myrosinase-treated glucoerucin (GER+MYR), which releases the isothiocyanate (ITC) erucin, on heme oxygenase 1 (HO-1) gene expression and Nrf2 signaling was investigated in vitro in cultured cells and in vivo in mice. Treatment of HT-29 cells with GER+MYR resulted in a significant increase in the mRNA and protein levels of nuclear Nrf2 and HO-1. GER+MYR was more potent at enhancing the nuclear Nrf2 levels than were the following myrosinase-treated glucosinolates: sinigrin, glucoraphanin and gluconasturtiin, which are the precursors of allyl-ITC, R-sulforaphane and 2-phenylethyl ITC, respectively. GER+MYR also significantly induced HO-1 gene expression in the mouse intestinal mucosae and liver but not in the brain. Mechanistic studies suggest that GER+MYR induces Nrf2 via ERK1/2-, p38- and JNK-dependent signal transduction pathways. The GER+MYR-mediated increase in HO-1 expression is primarily attributable to p38 signaling.

Organisationseinheit(en)

Institut für Lebensmittelwissenschaft und Humanernährung
Molekulare Lebensmittelchemie und -entwicklung

Typ

Artikel

Journal

Journal of Nutritional Biochemistry

Band

26

Seiten

661-666

Anzahl der Seiten

6

ISSN

0955-2863

Publikationsdatum

01.06.2015

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Endokrinologie, Diabetes und Stoffwechsel, Biochemie, Molekularbiologie, Ernährung und Diätetik, Klinische Biochemie

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1016/j.jnutbio.2015.01.004 (Zugang: Geschlossen)