Mucosal inducible NO synthase–producing iga+ plasma cells in helicobacter pylori–infected patients

verfasst von

Laura Neumann, M. Mueller, V. Moos, Frank Heller, Thomas F. Meyer, C. Loddenkemper, C. Bojarski, M. Fehlings, T. Doerner, K. Allers, Toni Aebischer, R. Ignatius, T. Schneider

Abstract

The mucosal immune system is relevant for homeostasis, immunity, and also pathological conditions in the gastrointestinal tract. Inducible NO synthase (iNOS)-dependent production of NO is one of the factors linked to both antimicrobial immunity and pathological conditions. Upregulation of iNOS has been observed in human Helicobacter pylori infection, but the cellular sources of iNOS are ill defined. Key differences in regulation of iNOS expression impair the translation from mouse models to human medicine. To characterize mucosal iNOS-producing leukocytes, biopsy specimens from H. pylori-infected patients, controls, and participants of a vaccination trial were analyzed by immunohistochemistry, along with flow cytometric analyses of lymphocytes for iNOS expression and activity. We newly identified mucosal IgA-producing plasma cells (PCs) as one major iNOS

⁺ cell population in H. pylori-infected patients and confirmed intracellular NO production. Because we did not detect iNOS

⁺ PCs in three distinct infectious diseases, this is not a general feature of mucosal PCs under conditions of infection. Furthermore, numbers of mucosal iNOS

⁺ PCs were elevated in individuals who had cleared experimental H. pylori infection compared with those who had not. Thus, IgA

⁺ PCs expressing iNOS are described for the first time, to our knowledge, in humans. iNOS

⁺ PCs are induced in the course of human H. pylori infection, and their abundance seems to correlate with the clinical course of the infection.

Organisationseinheit(en)

Abteilung Ernährungsphysiologie und Humanernährung
Institut für Lebensmittelwissenschaft und Humanernährung

Externe Organisation(en)

PathoTres Praxis für Pathologie, Berlin
Charité - Universitätsmedizin Berlin
Gastroenterologische Praxis am Rathaus Steglitz
Max-Planck-Institut für Infektionsbiologie
Robert Koch-Institut (RKI)

Typ

Artikel

Journal

The journal of immunology

Band

197

Seiten

1801-1808

Anzahl der Seiten

ISSN

0022-1767

Publikationsdatum

01.09.2016

Publikationsstatus

Veröffentlicht

Peer-reviewed

ASJC Scopus Sachgebiete

Immunologie und Allergologie, Immunologie

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.4049/jimmunol.1501330 (Zugang: Unbekannt)